Comparison of peanut stripe virus isolates using symptomatology on particular hosts and serology

-Twenty four isolates of peanut stripe virus from 8 d1fferent countnes were compared under controlled cond1tions at the Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). Montpelher, France. The isolates had thetr origins from Burma, lndia, Jndones1a, Phîhppincs, China. Tha1\and and USA. Four of the isolates were collected from Thaîland in 1972 but mamtamed m Japan and sent to France for th1s particular study By usmg disease react10ns on a set of groundnut gcnotypes and some other hast species, the isolates could be grouped into 8 strams that are mJld mottle. blotch, stnpe, blotch-stnpe, blotch-CP-N, chlorot1c rmg-mottle. chlorotic line-pattern and necrot1c strams. Sim1lanty was noted arnong isolates within the same strain groupmg regardless of their origins. Serological results ind,cated that peanut chlorotic-nng rnottle was an 1solatc of peanut stripe virus (PStV) but groundnut eyespot vtrus was a d1fferent virus. Serotypmg usmg serolog1cal reachons of viral antigens from lesional tissue of Chenopodium amarantrcolor to d1fferent polyclonal antibodies of different PStV isola tes and PStV-related viruscs. corrclated well wlth the grouping based on host d1sease react1ons. Similar tcsting usmg antigens from groundnuts was Jess effective in differentiating the strains Since ail the isolates sent from Japan are identical to PStV, it could be assumed that PStV has been m Southcast Asia as early as 1972 when the samples were collected frorn Thailand.